FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10787867
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT184894017.139069289600993No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1890901.752802477079111No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1230581.1407074262224406No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT866090.8028371132124635No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA861350.7984432881866267No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT713570.6614560598494587No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG677100.6276495622350554No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT650630.6031127376709409No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC528050.4894850854205006No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC484690.44929178307444834No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC482550.44730807304168657No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC401210.37190855245063736No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT397080.3680801774808681No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT371340.3442200390494247No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC366530.33976132631223577No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT311120.2883980679405855No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG291970.2706466440492824No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT280740.2602368012138081No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT270100.2503738690883008No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG246450.22845109232436772No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC241020.22341765985806092No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG239580.22208282693881934No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC232240.21527888691990735No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG218710.20273701928286658No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG200990.1863111586377548No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA200980.1863018889647045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199850.18525441591002187No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA163700.15174454783322783No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC163380.15144791829561857No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG161410.1496217927047117No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT145250.13464200105544497No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG140110.12987738910759652No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG115600.10715742046133864No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT111580.10343101189512255No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC108700.10076134605663938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2068600.084.750151
CGTTATC2106400.083.230452
GCGGTAT43850.081.4599461
TTATCCA2198300.079.822534
CACGCTG154600.078.843091
CGGTATC45150.078.785412
ATCCACC2297850.078.260126
TATCCAC2302350.077.73065
GTTATCC2286050.076.903553
CACCTTC2436000.073.598549
TCCACCT2443200.073.5208057
CCACCTT2463250.072.799878
ACGCTGC179400.068.1783452
GGTATCC53500.067.680563
AAGCAGT57200.066.764891
AGCAGTG61150.064.2870252
GCGTTAG3700.063.2867361
GACGTTT21350.062.9024163
GTATCAA62600.062.797969
GGAAGTT437250.061.855522
Produced by FastQC (version 0.11.5)