FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001600
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCA48450.018.9396461
CGGACTG45800.018.3058191
GACTAAC49300.018.1667441
CGGACGT59550.018.1438471
ATGCGAT45700.018.1051141
GGCTCGA36550.018.03181
GGGACCT41350.017.9076731
CTCGAAA32400.017.7605461
TGACTAG42950.017.7529161
GATCTAG44150.017.6940521
ACACCCT50800.017.6736431
CGCTGGA42250.017.6303751
GTAACTG48550.017.6088681
TGCCAAA46700.017.599621
TTGACTT40050.017.4999391
CCCTCCT63900.017.4596961
ACTGAAC29900.017.4424231
TGCTACC49900.017.4191171
GGAACTT41900.017.3574941
CTGAAAC36100.017.342221
Produced by FastQC (version 0.11.5)