FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10599570
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATTC774150.021.8171981
ATATTCA772400.021.7196392
TATTCAG775000.021.6910383
ATAAGAG287650.021.6476921
GCGAAGT362850.021.6429314
AGGGATC779350.021.6273218
TGCGAAG364750.021.6206553
GCGAGAA237500.021.6085721
TCAGGGA775850.021.6018586
GGGATCT780050.021.5811219
TTTACGT456450.021.55339810
CGAAGTC364450.021.5386585
CTTTACG457400.021.5329849
GGATCTG780750.021.53188510
GTAACCA344150.021.5118053
AAGACCT213450.021.5094131
ATTCAGG780450.021.4986974
GCTGCGA372200.021.4860821
AACCTCT458700.021.4830072
AGATCTC285600.021.4776863