FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10597523
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATTC773200.021.8152941
ATATTCA769200.021.7640442
TATTCAG774550.021.6869723
AGGGATC777950.021.6324778
ATAAGAG274700.021.5987531
GGGATCT778550.021.591799
TGCGAAG365350.021.591543
GCGAAGT365050.021.5731284
AAGACCT219950.021.571081
TCAGGGA775650.021.567796
ATTCAGG777250.021.5606924
GGATCTG779950.021.54093710
GCGAGAA243100.021.5333941
CTTTACG465800.021.5226299
TTTACGT465800.021.51945510
GCTGCGA371950.021.5105971
AACCTCT466600.021.4841252
CAGTAAC348600.021.4693641
AGATCTC288800.021.468583
TTCAGGG778500.021.4619875