FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17241823
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCA61650.018.848281
GGCTCGA45150.018.4492631
CGGACTG48250.018.2901781
CGGACGT75200.018.2469181
ATGCGAT51700.017.9635751
TGACTAG49550.017.8769361
GGGACCT45700.017.866051
GGAACTT45850.017.83161
GACTAAC55150.017.8175031
TGCTACC54250.017.7885571
AGAATGT44300.017.777738
CCCTCCT74850.017.7000941
CACCGAA41350.017.6628028
ACTGAAC36950.017.659611
ACCCACT51850.017.6144941
CGCTGGA48450.017.6014651
CTGAAAC43600.017.5656261
TGCCAAA52400.017.5346071
GATCTAG53600.017.4910431
TCCCGAT58800.017.4787541
Produced by FastQC (version 0.11.5)