FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15675792
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA59300.019.308968
GGGACCT43100.019.2935851
GATGCTA49600.019.2711011
GCCGAAG33250.019.0214923
CGCTGGA44900.018.74061
AAATGCC58850.018.597091
GACTAAC44600.018.5953711
ACGAGGA53000.018.574431
GAATGAA44550.018.5421731
GATGAAA55650.018.4014871
CCCTCCT72350.018.2588271
CATGACA63400.018.2513451
GGCTCGA37900.018.196871
TACTTGT59500.018.02421
CCACTAC48650.017.9969371
CGGCCGA27300.017.9694619
CTCGAAA44350.017.9065511
TGACTAG30600.017.9009571
AGAATAG62100.017.8894981
CCCGACA47700.017.87088810
Produced by FastQC (version 0.11.5)