FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9355730
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATTC673700.021.8107281
ATATTCA671350.021.7311972
ATAAGAG250250.021.6485041
TATTCAG676300.021.6327933
AGGGATC678150.021.6253388
TCAGGGA674250.021.600936
GGGATCT678950.021.5787989
AAGACCT191650.021.577391
GGATCTG679000.021.54458210
GCGAAGT321450.021.5436364
TTTACGT410200.021.52997210
GTAACCA297900.021.5298123
AACCTCT410150.021.5211722
TGCGAAG322950.021.5185013
AGTAACC298950.021.5119112
GCGAGAA215700.021.5054511
ATTCAGG678450.021.4961474
CTTTACG411350.021.4940749
GCTGCGA328600.021.4883921
CGAAGTC323000.021.4695245