FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9355730
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT149546315.984460859815321No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1597101.7070821838595172No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA995481.064032416497697No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA702200.7505560763296931No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT663880.7095972200993401No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT595980.637021376204743No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG543580.5810129193553042No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT509670.5447677519552189No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC410190.4384371930357118No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC369600.3950520162510034No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC368160.3935128525513242No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT319490.3414912572295267No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC311090.3325128023147312No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT287350.3071379785436305No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC281030.30038276008392717No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT242750.25946665840078753No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG235370.2515784444399315No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT219110.23419872099772013No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC203430.2174389384901018No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG199360.21308866331114729No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC198180.21182740416835458No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG189100.20212212195093276No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG168460.18006077558886371No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG165790.17720690956237514No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT163010.17423546853104996No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA161000.17208705253358103No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA132450.14157099446007954No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC130560.13955084210425056No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG127330.1360984124167756No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT122620.13106406448240812No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT122380.13080753719912824No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG118950.12714133477558673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102290.10933406586124225No Hit
GCGTTATCCACCTTCCACTGTACGTTGGCCTCTCTGGGATAGAAGTTATT99460.10630918164590043No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATCGAAGTTATT98650.10544340206483085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1772350.084.7314451
CGTTATC1803100.083.241762
GCGGTAT31950.079.8011551
TTATCCA1883950.079.7168054
CACGCTG133500.079.135931
CGGTATC31850.078.993172
ATCCACC1960700.078.343396
TATCCAC1963200.077.853965
GTTATCC1953450.077.0133363
CACCTTC2081050.073.59669
TCCACCT2089050.073.401467
CCACCTT2102950.072.820078
GCGTTAA7850.069.405441
ACGCTGC154800.068.4154742
GGTATCC38100.067.261973
AAGCAGT57700.067.130331
AGCAGTG59350.065.950212
CGTTAAC7800.065.382862
GTATCAA60350.065.069029
GCGATAT6400.063.182511
Produced by FastQC (version 0.11.5)