FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15438529
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA55800.019.2787348
GCCGAAG33800.019.168123
GGGACCT43850.019.1150171
CGCTGGA45200.019.1038361
GATGCTA49950.019.0709251
GATGAAA57600.018.5814551
AAATGCC59700.018.5358721
GAATGAA43800.018.4838751
GACTAAC43800.018.4838751
GGGAATG54300.018.4749951
CCCGACA47200.018.43352310
AGAAGAA22400.018.217968
CCACTAC48850.018.216851
TCCCGAT41650.018.1439191
AGAATAG60250.018.074551
GGCTCGA41400.018.0674951
ACGCCGA66950.018.0566221
CGACCTT59800.018.0450121
ACGAGGA49900.018.0319271
CATGACA64000.018.0123621
Produced by FastQC (version 0.11.5)