FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5679088
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATTC407150.021.7937891
ATATTCA404650.021.784172
TATTCAG406750.021.7137993
AGGGATC408800.021.6794838
GCGAAGT195650.021.637944
ATAAGAG152800.021.6254751
TGCGAAG196200.021.59413
AAGACCT118950.021.5846791
TCAGGGA407950.021.5823336
GGGATCT410000.021.581169
CTTTACG242800.021.5530289
TTTACGT242550.021.54350310
ATTCAGG410000.021.5014384
GGATCTG411250.021.49416710
AACCTCT243500.021.4863382
GCGAGAA131050.021.4828341
GCTGCGA199900.021.4781441
GCCGAGA100400.021.4718884
CCATTTG180700.021.4674197
CGAAGTC196650.021.4661925