FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15438529
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC14225889.214530736704255No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC8386345.432084883216529No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC7166454.64192540623527No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC4088712.648380554909085No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1572731.0187045669959878No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1028490.6661839350109068No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC801460.5191297694229806No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT524170.33952068879101116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391070.25330781190358226No Hit
CTGATGGCTCAAACACATCGACCTCGGGTGGGAACACGTTTTTCAGGTCC309720.20061496791566086No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG273680.17727077495530824No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA265650.1720695022174716No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG249810.16180945736475283No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTC245960.15931569646305033No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA244140.15813682767315462No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG226210.14652302690236876No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG209110.13544684211818367No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT204750.13262273886326864No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGACCACGTTTTTCAGGTCC196290.12714294218056657No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT187600.12151416757386665No Hit
TCTGATGGCTCAAACACATCGACCTCGGGTGGGAACACGTTTTTCAGGTC186570.1208470055663982No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT184240.11933779442328994No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA175520.11368958791345989No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA174210.11284106147677671No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT173250.11221924057661192No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT172350.11163628348270745No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA170110.11018536804898965No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG169060.10950525143943442No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT168850.10936922811752338No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG159440.1032740878356999No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA154560.10011316492652894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1524150.080.3525161
TCTGATG1612900.079.4958041
GTCTCTC1819100.067.284392
TCTCTCA1894400.065.599163
CTCTCAG2225400.055.758094
TCAGCTG2234150.055.0965467
AGCTGGT2238900.054.822129
TCTCAGC2265650.054.6775055
CTCAGCT2270100.054.392496
CAGCTGG2278450.054.258438
CTGATGG4256300.052.1230281
TGATGGC4398200.050.3263972
GATGGCT4646000.047.4501043
TGGCTCA4716050.047.0147255
ATGGCTC4724300.046.850774
GGCTCAA4783200.046.8175626
GCTCAAA4808800.046.60197
CTCAAAC4834750.046.517898
CTTCCGA13350.046.4734344
TCAAACA4879500.046.2384579
Produced by FastQC (version 0.11.5)