FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367382
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATA250.002576309722.0010911
AAAGATT250.002576309722.0010911
GTTTCTA950.021.9980951
GGAGATT302.8691898E-421.9980952
AAATAGC302.8691898E-421.9980954
GCTGGCA656.730261E-1121.9980953
AGAAGAA504.606227E-821.9980958
CACGCGA750.021.9980958
GGGAGAT504.606227E-821.9980951
CCTAAGG302.8691898E-421.9980954
TATTGGA403.6069196E-621.99809510
CTAACAC353.2096497E-521.9980954
TAATTGG353.2096497E-521.9980959
GGGAATG1250.021.9980951
TCTAACC353.2096497E-521.9980958
GGAAATA656.730261E-1121.99809510
CCGAATC504.606227E-821.9980953
ACTTTAG302.8691898E-421.9980953
ACGAGGA1150.021.9980951
TGAGCAT800.021.9980951
Produced by FastQC (version 0.11.5)