FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351961
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTAC656.730261E-1122.0000028
TCTTGAG353.2070107E-522.08
AGATATG403.6034999E-622.08
TAAAGTA403.6034999E-622.05
GGATGAT302.8671784E-422.02
GACACGA250.002576838722.09
AAAAGCT250.002576838722.05
CACGCCG353.2070107E-522.011
TAACGCA504.600588E-822.02
CCGCTAG302.8671784E-422.010
AGTTGTA250.002576838722.08
TTGACTT504.600588E-822.01
TTGAAGA353.2070107E-522.04
GGAAACA353.2070107E-522.010
CCCTTGA250.002576838722.011
GGGTTGC750.022.01
TAGGCTA403.6034999E-622.07
TGTAATG353.2070107E-522.09
TAATGTA353.2070107E-522.04
CCACCTA1150.021.043481
Produced by FastQC (version 0.11.5)