FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8961915
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA98850.021.1542681
ACTGAAC81900.020.9365251
GCGACCA96050.020.8657321
GTAACTG87000.020.8224411
TCGCAAG89050.020.6897268
TGAGCCG86100.020.6694031
GAGCAGA105250.020.5999031
GAAGCCC85100.020.5643259
CTACAGT98700.020.5424543
CTGGTGT78300.020.5273363
AGGCCAC100050.020.5155331
GACTACA101450.020.4819361
TTTGTCA85100.020.4725721
GGCGACT50200.020.4330521
TAACTGC59350.020.4084842
AGAATGT51550.020.377398
GGCCACT77800.020.376452
TACTTCT89200.020.3713159
ACTTCTT89250.020.35990110
TTAGTTC80950.020.3528861
Produced by FastQC (version 0.11.5)