FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351961
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3709510.539520003636767No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC218086.196141049718577No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC185745.277289245115226No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC106283.019652745616702No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC41951.1918934200096032No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT27570.783325425260185No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC20480.5818826517710769No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT14000.39777134398413466No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7460.21195530186583172No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG6940.19718093766070674No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG6490.18439543017550242No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTC6280.17842886001574038No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA6220.1767241256843798No Hit
CTGATGGCTCAAACACATCGACCTCGGGTGGGAACACGTTTTTCAGGTCC6130.17416702418733895No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG6080.17274641224453846No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG5720.162518006256375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5340.15172135549109134No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT5090.14461829577708893No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA4840.13751523606308655No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT4660.1324010330690048No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA4640.1318327882918846No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT4630.1315486659033245No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA4510.12813919724060338No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT4390.12472972857788221No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT4390.12472972857788221No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG4380.12444560618932211No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG4320.12274087185796155No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA4200.11933140319524038No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG4130.11734254647531972No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG4110.11677430169819952No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA4060.11535368975539903No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT4060.11535368975539903No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCGAGCGTG3990.11336483303547835No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTATG3960.11251246586979809No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG3880.1102394867613173No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA3830.10881887481851682No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC3800.10796650765283652No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC3760.10683001809859616No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG3720.10569352854435576No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT3590.10199993749307452No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC3570.10143169271595433No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG3570.10143169271595433No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG3530.10029520316171393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG40100.080.7502061
AGTCTCT35350.080.661471
TCCGATC555.456968E-1269.536516
GTCTCTC42650.066.955042
TCTCTCA44700.064.930123
CTTCCGA501.3395038E-859.492344
CTCTCAG50450.057.1086044
TCAGCTG50550.056.911577
AGCTGGT50350.056.8000349
CTTATGG302.2545099E-456.6593741
TCTCAGC50950.056.4647645
CAGCTGG51400.055.8050738
CTCAGCT51650.055.617246
GGCGTTT552.8414433E-854.083954
GCGTTTG552.8414433E-854.083955
CTGATGG103900.051.369711
GAAGGCG509.131054E-750.9934351
TTCCGAT605.6405042E-849.5769545
CGTTTGC605.6405042E-849.5769546
TGATGGC108500.049.1918262
Produced by FastQC (version 0.11.5)