FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18306337
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA67550.019.3278968
GCCGAAG39900.019.1871743
GGGACCT54050.019.068591
GATGCTA57400.019.047991
AAATGCC69000.018.4919871
AGAAGAA26900.018.440968
AGAATAG72700.018.3830071
GATGAAA70250.018.3821561
GGGAATG69250.018.3616921
ACGAGGA62250.018.3414081
CGCTGGA54400.018.3393141
GACTAAC51450.018.2577461
CCCGACA56250.018.22437510
GAACCTA47700.018.1480941
CCCTCCT80250.018.133831
GAATGAA52700.018.0960241
CGACCTT69350.018.0179981
GGCTCGA46550.017.9584391
CGGCCGA28550.017.9145589
CATGACA72750.017.8714241
Produced by FastQC (version 0.11.5)