FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147566
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA65700.019.236728
GCCGAAG38500.019.199493
GGGACCT57050.019.050291
CGCTGGA51350.018.8085081
GATGCTA59600.018.5674361
GACTAAC52900.018.5069391
CCCGACA59050.018.44130310
CATGACA75350.018.3068871
AAATGCC66700.018.256691
AGAATAG73350.018.2211761
ACGCCGA77750.018.1945291
GATGAAA67050.018.1942021
GGCTCGA46650.018.1803821
GGGAATG66400.018.1569421
GAATGAA52900.018.1326431
ACGAGGA62200.018.074271
CCACTAC55050.018.0638941
CGGCCGA28850.017.9958579
GAACCTA49200.017.9535661
CCCTCCT83450.017.9403971
Produced by FastQC (version 0.11.5)