FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147566
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC17671799.737829304491854No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC10492905.7819875128157685No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC8892624.900172287567379No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC5130612.827161504743942No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1970621.0858866693197313No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT1283480.7072463602005911No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC1003860.5531650911202086No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT648300.3572379899320934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG441700.2433935217538264No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG343650.18936423760629936No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA341240.18803623582358098No Hit
CTGATGGCTCAAACACATCGACCTCGGGTGGGAACACGTTTTTCAGGTCC324420.1787677752487579No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA323340.1781726541179131No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG313700.17286064698703946No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGCTTC307270.16931747210617668No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG287320.15832426232807198No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT270550.14908335365745468No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG265140.1461022376223897No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT240930.13276160560595288No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCTGGCTTT237430.13083297231154856No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT222580.12265005676243305No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA221750.12219269515261716No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA220430.12146532488158467No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT219280.12083163108485183No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT218760.12054509128111175No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG218540.12042386290260633No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTTTA218510.12040733176008286No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG199980.11019659606142224No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATACGGATGA198300.10927085208010814No Hit
TCTGATGGCTCAAACACATCGACCTCGGGTGGGAACACGTTTTTCAGGTC197050.10858205447496375No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC192180.10589849900532115No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG190320.10487356816886628No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG187910.10354556638614788No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA187630.10339127572259552No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGAGTTCC184910.1018924521338013No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC183980.10137998671557386No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG183370.1010438534842634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTCT1810500.080.226821
TCTGATG1957850.079.380631
GTCTCTC2164750.067.025492
TCTCTCA2248400.065.459853
CTCTCAG2635150.055.718734
TCAGCTG2653450.054.807577
AGCTGGT2652600.054.5640079
TCTCAGC2687000.054.5375865
CTCAGCT2696250.054.1408656
CAGCTGG2699200.053.9022258
CTGATGG5091800.051.6138951
TCCGATC16700.050.891696
TGATGGC5248900.049.9824562
TTCCGAT17300.049.372295
CTTCCGA17800.048.7016334
GATGGCT5549450.047.1476063
TGGCTCA5624800.046.787235
GGCTCAA5695800.046.573326
ATGGCTC5639800.046.566354
GCTCAAA5744200.046.3466077
Produced by FastQC (version 0.11.5)