FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21298057
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA79200.019.3598798
GCCGAAG46050.019.1566663
GATGCTA69750.018.987051
GGGACCT60750.018.9753681
GATGAAA79150.018.6777081
CGCTGGA62650.018.6632561
GGGAATG77500.018.3515191
CATGACA86500.018.349551
ACGAGGA74050.018.3152661
GGCTCGA57300.018.3133981
AAATGCC77900.018.2290481
GACTAAC65150.018.2003421
ACTGATG62900.018.1344051
CGACCTT82300.018.0964781
GAACCTA58000.018.0923771
CCCGACA65000.018.0388510
CGGCCGA34400.017.937819
TACTTGT73650.017.9218861
AGAAGAA31050.017.8893598
AGAATAG83850.017.880021
Produced by FastQC (version 0.11.5)