FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535208
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA129200.021.2183931
ACTGAAC101450.021.1427291
GCGACCA126650.021.037341
TGAGCCG116400.020.8760031
TCGCAAG117100.020.7967788
GGAAATA74800.020.7491710
GAAGCCC108350.020.6692249
GCAAAGA89300.020.61955610
CTACAGT130650.020.5797713
TGTACGA94650.020.54645710
GAGCAGA130050.020.538121
TTTGTCA107500.020.5363731
CGCAAGC118700.020.5071839
GGAGCAA74650.020.492091
GTAACTG111550.020.4912381
TCGCGTA108700.020.4815984
GATGAAA95250.020.4738731
GGCCACT96450.020.440172
GGTGAAG88450.020.4303861
AGGCCAC129250.020.4183141
Produced by FastQC (version 0.11.5)