FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535208
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT124658310.806766553320928No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1642941.424282943142421No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1132690.9819415479980942No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1030860.893663989413975No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC909620.7885596861365656No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT749360.6496285112500789No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC644720.5589149324398831No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA591770.5130119890339212No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT530540.459931021616602No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC485090.42052991155426067No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC421260.3651949752444863No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG395630.3429760434315532No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT368610.3195521051722691No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG345710.299699840696414No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC301340.26123499463555405No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG287910.24959237839491058No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT248720.21561813189671136No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC240910.20884755610822103No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG204370.17717062405810108No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT177930.15424949424405698No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT168530.14610052978671906No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC153580.13314020865510184No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG145630.12624826531086394No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGGTATT131840.1142935610697267No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGCTAGAAGTTATT127750.11074789461967222No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG121010.10490491372153844No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA120980.10487890638816397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1672850.084.609641
CGTTATC1700100.082.933962
TTATCCA1778700.079.6422044
GTTATCC1819650.078.3684163
GCGTGAT22950.077.431481
TATCCAC1916400.077.1779565
GCGGTAT83250.076.9067841
ATCCACC1952150.076.701366
CACGCTG146950.075.9712141
CGTGATC23200.075.8417362
CGTTAAC7250.075.621912
CGGTATC84000.075.3881152
CACCTTC2016900.074.018039
CGTTAGC6900.073.298282
GCGTTAA7850.073.111891
CCACCTT2112050.070.7518548
TCCACCT2118900.070.647497
TAGCTGC579950.067.418581
TTCTCGT8650.066.350111
GGTATCC102500.066.0920263
Produced by FastQC (version 0.11.5)