FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20947527
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA76850.019.480118
GCCGAAG46150.019.4728533
GATGCTA69400.019.1315361
GGGACCT63750.018.9980581
CGCTGGA59400.018.6485521
ACGAGGA68700.018.5098611
GGCTCGA54150.018.3845141
GACTAAC62200.018.3396241
AAATGCC74900.018.3288331
ACTGATG62850.018.3249781
GAATGAA59800.018.266281
GATGAAA79050.018.2293621
GGGAATG74700.018.2159231
AGAAGAA29200.018.1568858
GAACCTA57050.018.1248441
AGAATAG81550.018.1156581
CATGACA89400.018.0753351
CGGCCGA30500.018.0321439
CTGCGGA58500.017.9388481
CCCGACA64600.017.91267410
Produced by FastQC (version 0.11.5)