FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8186283
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA94700.021.163311
GCGACCA89000.021.0842441
ACTGAAC75200.021.06041
GGAAATA56800.020.83714310
GTAACTG78700.020.7251221
AGGCCAC87000.020.7213611
TGAGCCG78950.020.7152581
CTACAGT92000.020.62743
TGTACGA68600.020.62011510
GGCCACT68550.020.6064622
TAAGAAA27900.020.5403466
GGCGACT43250.020.5357741
ACTTCTT81150.020.53517210
GAAGCCC77200.020.4887689
CTGTACG69200.020.4572249
TCGCAAG86300.020.4568378
TACTTCT81650.020.4498379
GAGCAGA89800.020.4061721
ATTCGCG81150.020.402992
CGCAAGC86650.020.3742079
Produced by FastQC (version 0.11.5)