FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14889450
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG45900.019.3996661
GGGAGAT45100.019.1084631
TACGGAT54100.018.7407131
GGAAAGC37200.018.574631
GAACCTA50600.018.5342331
GATGCTA44450.018.4457681
AGAAACC49150.018.4157188
GGCGACT47700.018.4134651
AAACCTG46450.018.3633021
AGTGAAC40100.018.0739618
CGTGAGC41050.017.9869371
CTGATAG54750.017.9747521
AGCTGAA32950.017.9572248
CGATTGA40550.017.9097731
TGGACGA31350.017.8914269
AGCTTGA29950.017.8091871
GGCTCGA36400.017.8021131
CTAGCCT55450.017.7875881
CCTTACG50150.017.7116781
GACTAAC40200.017.681911
Produced by FastQC (version 0.11.5)