FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13151285
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA148150.021.2528271
ACTGAAC118100.021.0507131
GCGACCA138650.020.946911
GAAGCCC120750.020.7601939
GAGCAGA151350.020.6653211
TCGCAAG135050.020.6551748
CGCAAGC135250.020.6246329
TGTACGA109600.020.62412310
TGAGCCG130100.020.623241
GACTACA150400.020.6202371
AGGCCAC146550.020.5987341
CGAAGTA98050.020.5631245
CTACAGT140850.020.542383
GGAAATA91950.020.53963910
GCGACTA71700.020.5299912
GTAACTG124350.020.523691
TACTTCT124250.020.4675399
GGCCACT114100.020.4602812
GATGAAA110600.020.4383491
TTTGTCA118900.020.4370271
Produced by FastQC (version 0.11.5)