FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11122038
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT35800.019.1764741
TGACTAG35150.019.1548861
GAACCTA37750.018.5653841
AAACCTG34750.018.3923381
AGCTGAA23000.018.266248
GGGAGAT34600.018.2491341
TACGGAT42100.018.0867141
AGAAACC37800.018.0099498
CTGATAG40950.017.9488091
AGTGAAC28700.017.9340158
CTGAAGT32200.017.9324281
GGAAAGC28900.017.9210621
CCTTACG36300.017.8802011
GACTAAC27350.017.8488461
TGCCCAT35950.017.8090571
TTTATGC36100.017.796111
TCTAACT36900.017.7338988
CGATTGA29450.017.6986271
GACCAAT39500.017.6312751
ATGCGAT31450.017.6242681
Produced by FastQC (version 0.11.5)