FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13062856
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA150850.021.2101881
ACTGAAC120600.021.0545061
GCGACCA144200.020.9518261
TGAGCCG130300.020.8301981
TGTACGA109850.020.6673610
GAGCAGA150800.020.6479241
CTGCCTA76300.020.6423841
GACTACA154300.020.5932851
GTAACTG122400.020.5830211
GAAGCCC124600.020.5778339
TCGCAAG134450.020.542888
AGGCCAC148550.020.5012911
GGCCACT112150.020.4814072
TTTGTCA121100.020.4767861
ATGAAGT153600.020.4691413
TACTTCT128000.020.4609039
GGAAATA88950.020.44100610
AGAAGCC125950.020.39228
TAAGAAA50600.020.3904916
ATTCGCG127150.020.383912