FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13062856
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT180796313.840487868809086No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2601031.9911648723678805No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2151921.647357974397023No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1334381.0215070884958082No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1301870.9966197284881653No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT1157110.8858016960456427No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC985410.7543603022187491No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA922220.7059865009611986No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT809440.6196500979571389No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG738110.5650448875804801No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC708780.5425919109879187No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC602730.46140752068307267No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG490040.375140015322836No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC442370.3386472299778854No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG365980.2801684409596186No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT364880.2793263586462256No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT331260.25358926103143137No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC305580.23393046666058326No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG288880.22114612608452547No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC224810.17209865897626062No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA218930.16759734624648698No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG193740.14831366126978662No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT160170.12261484012378303No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG158010.12096129667202946No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA156900.12011155906487832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2069050.084.564531
CGTTATC2104350.083.064912
TTATCCA2203650.079.364714
GTTATCC2242400.078.0725563
CACGCTG164800.077.958471
TATCCAC2290850.077.240195
ATCCACC2319600.076.5329066
CACCTTC2394850.073.725849
CGTTAAC5600.073.6176152
TTCTCGT10500.071.260591
CCACCTT2505900.070.4552468
TCCACCT2513000.070.445617
TAGCTGC696450.068.7466661
CGTTAGC2500.068.001432
GCGTTAA7200.066.722451
CGTAATC6250.066.6413962
GCGTGAT5400.066.131991
CGATATC7000.065.572812
ACGCTGC199500.064.315882
GCGGTAT29900.063.5568541
Produced by FastQC (version 0.11.5)