FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10685680
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG32750.019.482981
GGCGACT30900.018.7235811
CTGAAGT31250.018.6549361
GAACCTA37050.018.4710481
GGAAAGC26800.018.4628891
GATGCTA30250.018.39731
GGGAGAT31600.018.3785671
TACGGAT38400.018.3382451
ATGCGAT33300.018.2676621
CGTGAGC28850.018.2587151
AGAAACC37450.018.2076248
CGATTGA27750.018.1882361
GGCTCGA29350.018.1729491
AGTGAAC28100.018.1603878
GACTAAC28650.018.1553861
CCTTACG37600.018.1129321
AAACCTG34550.018.0851921
AGCTGAA23350.018.0395348
CTGATAG40950.017.9229071
AGAATAG34550.017.830021
Produced by FastQC (version 0.11.5)