FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9566523
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT418600.021.799141
TTTACTG438600.021.7458921
GTACTGT415200.021.6526153
CGTACTG415750.021.629352
TACTGTA417750.021.6047044
AGCTAAT359800.021.5827272
CTGTTTA354450.021.5442521
TTTAGTA345150.021.5120644
GTAGCTA353200.021.4988238
TTAGTAG345950.021.4851365
AGAGCGA211850.021.4562441
GTACTCG422600.021.4432138
ACTCGCG421650.021.4331210
GCTAATC362550.021.4323083
GTTTAGT348000.021.418073
CGCACTC364450.021.416719
TGTTTAG348400.021.3982642
AGTAGAA207300.021.3953197
CCGAATC170350.021.3926943
TCGCACT364600.021.3908048
Produced by FastQC (version 0.11.5)