FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11096245
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT36550.018.9632871
TACGGAT42100.018.8713761
TGACTAG35050.018.8002451
GGGAGAT31700.018.5623251
GACTAAC28200.018.4825731
GAACCTA38600.018.3558311
GATGCTA33300.018.2660751
AGCTGAA24350.018.2021228
AGAAACC39000.018.1890938
GGAAAGC26750.018.1662461
AGTGAAC30150.018.1659438
CTAGCCT39100.018.0083731
TTTATGC36800.017.9062141
CCTTACG36700.017.8949551
CTGATAG41950.017.8356191
CGATTGA30050.017.7847441
CTGAAGT30050.017.7480751
GGCTCGA28050.017.717161
ACTTGAC24200.017.67869210
CTTAGGA28800.017.6383861
Produced by FastQC (version 0.11.5)