FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531847
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT409400.021.7668481
TTTACTG441400.021.6771
GTACTGT405450.021.6248473
TACTGTA407050.021.5830824
CGTACTG406500.021.5577372
CTGTTTA372950.021.5487541
AGCTAAT358100.021.5461712
TTTAGTA363200.021.5116424
GTAGCTA372300.021.4896838
TTAGTAG363800.021.486815
AGAGCGA207700.021.4815861
GTACTCG412750.021.4598248
ACTCGCG412050.021.45789110
TGTTTAG365550.021.4351582
CGCACTC359950.021.434469
GTTTAGT365700.021.4337643
TACTGGT445950.021.4171263
AGTAGAA203800.021.4071067
GCTAATC360650.021.4044953
AGTAGCT366700.021.397887
Produced by FastQC (version 0.11.5)