FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9531847
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT124117313.021327346106165No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT2053172.1540106550178577No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1719271.8037112849167636No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1300251.36411127874797No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC982921.0311957378249987No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT876530.919580433886528No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT852110.8939610549770679No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT684900.7185386001265022No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA636820.6680971694153295No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT612580.6426666311366517No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG470730.49384972293407564No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC457380.47984404281772464No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT406690.42666442296020907No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG394140.4134980345362237No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC335770.3522612144319983No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC334400.35082392740882223No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT332340.34866275130098084No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG315290.3307753471074389No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG309620.32482686723779763No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT302410.31726275086035266No Hit
TAGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT229380.24064591049352763No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG207790.21799552594581095No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG206930.21709328737651792No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC185460.19456879658265602No Hit
CTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC141020.14794614307174675No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC137580.14433718879457466No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC129170.13551413487858124No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG119800.12568393093174912No Hit
AGCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC113340.11890665051589686No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA112330.11784704475428529No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA102010.107020181922769No Hit
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG100870.10582419126114802No Hit
GCTGCTGGCCGCGTACCTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT100330.10525766936880124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1341400.084.7096251
CGTTATC1363000.083.381222
CACGCTG201300.082.136271
TTATCCA1438450.079.102224
ATCCACC1487050.076.931376
TATCCAC1488400.076.687425
AAGCAGT66050.074.4090651
GTTATCC1563600.072.749173
CACCTTC1590600.071.63229
AGCAGTG69650.071.020842
TGATACG2650.070.560363
TCCACCT1631900.069.9391257
CCACCTT1651250.069.060368
GCGATAT3200.065.100881
ACGCTGC255400.064.8264162
TAGCTGC563250.060.1586341
GGAAGTT189550.059.5467722
GTATCAA86000.058.3589829
GCGTTAA3700.057.4525221
CGTTAAC3350.055.8164022
Produced by FastQC (version 0.11.5)