FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15726135
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT672850.021.7723391
TTTACTG738250.021.7185341
AGCTAAT598450.021.6723882
TACTGTA669850.021.5755984
GTAGCTA592450.021.558898
TTTAGTA580200.021.5442584
GTACTGT669250.021.5407033
CTGTTTA598850.021.4990731
CGCACTC607400.021.4946929
CGTACTG670250.021.4931742
AGCTAAA593900.021.49061610
TTAGTAG581450.021.4880965
GCTAATC604100.021.4857853
TCGCACT607700.021.475858
AGAGCGA341750.021.4588491
ACTCGCG677600.021.43989610
TAGCTAA596050.021.4297059
TGTTTAG586000.021.4192962
GTACTCG681000.021.3918198
CAGCTAA618000.021.3901541
Produced by FastQC (version 0.11.5)