FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11904142
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG37300.019.3818761
GGCGACT38400.018.9414621
AAACCTG38000.018.792831
GGAAAGC30700.018.6307241
AGCTGAA25300.018.5182788
GACTAAC28300.018.4972821
GGGAGAT38400.018.3674771
AGAAACC41800.018.3386768
GAACCTA37150.018.3328691
CCTTACG40050.018.1611021
GATGCTA36150.018.1083491
TACGGAT41150.018.0237851
GGCTCGA28950.018.0058381
CCTCTGA41300.017.9583241
CGTGAGC39400.017.9012971
CTGATAG44350.017.8663581
CTGAAGT31850.017.7850231
AGCTTGA24650.017.749021
AGTGAAC33100.017.7429058
ACTGAAC26200.017.6243671
Produced by FastQC (version 0.11.5)