FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9396842
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT414800.021.750791
TTTACTG442050.021.7342911
TACTGTA410650.021.6321374
GTACTGT410300.021.586253
AGCTAAT351600.021.5577832
CTGTTTA359550.021.539861
TTTAGTA349850.021.5274894
CGTACTG411500.021.5244432
GTAGCTA358950.021.5093778
TGTTTAG352400.021.480742
CGCTTAG170750.021.4646519
CGCACTC354350.021.4436879
GTTTAGT353500.021.4234523
AGAGCGA206950.021.4179041
GCTAATC353850.021.4178073
TCGCACT355050.021.4048488
CGGGCGT241000.021.3966353
ACTCGCG417000.021.39263710
TTAGTAG352250.021.3907535
CAGCTAA363100.021.3807871
Produced by FastQC (version 0.11.5)