FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9374097
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT406300.021.8041861
TTTACTG445650.021.7258261
TACTGTA404800.021.6002244
AGCTAAT345850.021.5949612
GTACTGT404100.021.5886463
CTGTTTA358300.021.531291
GTAGCTA355200.021.526448
TTTAGTA347300.021.5182534
CGTACTG405650.021.5040252
GTTTAGT349300.021.4706253
TTAGTAG348050.021.4692975
TGTTTAG349850.021.4518552
TCGCACT349250.021.4364768
AGCTAAA357150.021.4107310
GCTAATC349050.021.410373
AGTAGAA191950.021.4042667
CGCACTC349950.021.3986289
CCGAATC172600.021.3942383
AGTAGCT350800.021.3856587
TACTGGT451300.021.3854583
Produced by FastQC (version 0.11.5)