FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14132498
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA58750.019.3806381
GGCGACT52500.018.9428231
GACTAAC49250.018.874961
GGGACCT36100.018.4976711
ACTGATG46200.018.287521
GATGCTA44100.018.2852541
TGACTAG47650.018.2620331
AGCTTGA40200.018.2530481
ATAACGC36250.018.2086941
ACTGAAC43400.018.1999531
GATGAAA40500.018.1993271
CAATGGA42550.018.1451028
CGGACTG36750.018.1106321
GAACCTA40400.018.1082231
CCTTACG47900.018.0518881
CCTCTGA49100.018.0364061
TCTGGAA41100.017.9871771
GATCTAG41950.017.9374081
CGTCTAC56900.017.9226841
TGATTTC30750.017.8879451
Produced by FastQC (version 0.11.5)