FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17761897
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC60150.018.9122221
GGCGACT63700.018.7563361
GGGACCT45250.018.5751511
CTCGAAA70950.018.4213581
GATGAAA45600.018.3843271
GATGCTA53950.018.2511061
ACTGATG58250.018.1692391
GGAACTT50650.018.1369651
TGACTAG58300.018.0970441
AAGAAAA22950.018.021032
CGGACTG42150.017.9576071
CCTTACG60300.017.9346871
TTGACTT76250.017.9200551
GATCTAG51900.017.890921
CATATTC62300.017.8357321
ACTGAAC52350.017.8211941
GAACCTA49950.017.7964551
CAATGGA54750.017.7780728
AGCTTGA49700.017.7531571
GGAATAA63150.017.7524571
Produced by FastQC (version 0.11.5)