FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403139
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGG481000.021.8540231
ATCTACT672050.021.8465291
GTTCTCG450750.021.8321
GCAATTG564500.021.8070682
CTAACTT426550.021.7889211
AGTGAGG371850.021.7430211
AGTTATC284600.021.7216973
TCTACTT675100.021.7197272
TCGGTAC441850.021.7186645
CGGTACC441650.021.7135566
GCAGTTA288550.021.7106571
CTCGGTA442450.021.7066154
GTACCCA442400.021.6991238
GGTACCC443050.021.6697717
ACCCAAT442550.021.66194210
TACCCAA443750.021.6380679
CAGTTAT284800.021.6330592
CTACTTC679750.021.6294043
GGCAATT574500.021.628931
ACTTCAT680700.021.6202245
Produced by FastQC (version 0.11.5)