FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5511751
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT697850.021.8808921
AGAGTGG490350.021.8391671
GTTCTCG469300.021.824891
GCAATTG565250.021.790512
CTAACTT428600.021.7491651
TCTACTT702450.021.7235072
AGTGAGG383200.021.7164961
GCAGTTA296950.021.711741
CTCGGTA463000.021.7031124
CGGTACC462950.021.7030816
TCGGTAC463300.021.6914355
GGTACCC463650.021.6796077
GTACCCA464000.021.6774798
CTACTTC705150.021.6771773
GGCAATT573300.021.655311
TACTTCA705800.021.6494244
AGTTATC293850.021.6444663
CAGTTAT294150.021.637942
ACTTCAT707150.021.6360935
TCTCGGT464800.021.6332653
Produced by FastQC (version 0.11.5)