FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7160018
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT889200.021.8477921
AGAGTGG630300.021.8446271
CTAACTT561950.021.8139921
GCAATTG736450.021.8132462
GTTCTCG599900.021.7982521
CTCGGTA590250.021.7446374
AGTGAGG504850.021.734131
TCTACTT894450.021.7146362
GTACCCA592300.021.7085298
GCAGTTA393900.021.7039381
AGTTATC387350.021.7017733
TCGGTAC591600.021.6857195
CGGTACC591600.021.6838596
CTACTTC897650.021.6727643
GGTACCC592600.021.6678377
GGCAATT747300.021.6599271
TACCCAA594600.021.6356589
TCTCGGT593050.021.6308423
ACCCAAT593850.021.62778710
CAGTTAT388700.021.62642
Produced by FastQC (version 0.11.5)