FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7289085
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGG650250.021.8613111
ATCTACT908950.021.8552861
GTTCTCG617950.021.8237081
GCAATTG742100.021.8208772
CTAACTT567000.021.7824861
TCTACTT911950.021.7604682
AGTGAGG517150.021.7166841
CTCGGTA607000.021.7154054
GCAGTTA385600.021.6764971
GTACCCA608000.021.6723028
CTACTTC918250.021.667493
AGTTATC382450.021.6611213
GGCAATT753700.021.6543481
CGGTACC608550.021.6510586
GGTACCC608550.021.6509087
ACTTCAT920150.021.649655
TACTTCA919850.021.6495364
TCTCGGT608800.021.6463743
TCGGTAC608600.021.6456645
ATCATTC923300.021.62806110
Produced by FastQC (version 0.11.5)