FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14728537
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACT53700.019.0722791
GACTAAC49300.018.8554551
CTCGAAA60100.018.670371
GATGAAA42400.018.6028941
ACTGAAC42350.018.5209521
ACTGATG49200.018.5136661
GGAACTT44400.018.4338971
AGCTTGA43100.018.428381
CCTTACG52800.018.3139551
CCTCTGA51700.018.150381
GGGACCT34850.018.087521
TGACTAG48900.018.0648291
TTGACTT64200.018.0606251
ATCGAGT49600.018.0538541
TCTGGAA44050.018.0309451
GAACCTA41000.017.977041
CGGACTG37150.017.9152871
CATCAAG65300.017.8743131
CCGGGAT44150.017.8655191
ATAACGC41150.017.8313061
Produced by FastQC (version 0.11.5)