FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393010
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT1180750.021.8726481
AGAGTGG838750.021.840281
GTTCTCG789900.021.8262061
GCAATTG981200.021.8253922
CTAACTT737450.021.8123361
TCTACTT1188250.021.7318152
CTCGGTA774900.021.7205164
AGTGAGG653900.021.7092551
GTACCCA776650.021.691178
GCAGTTA503950.021.6881451
CGGTACC776000.021.6839396
AGTTATC497550.021.6752853
CTACTTC1193700.021.6666933
TCGGTAC777000.021.660285
ACTTCAT1197450.021.6555655
GGTACCC777700.021.650577
GGCAATT996800.021.6471481
TACTTCA1196600.021.6379684
TAACTTC739700.021.635942
GAGTGGG836750.021.6269282
Produced by FastQC (version 0.11.5)