FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393010
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT218667923.279853848766262No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2752042.9298808369202205No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA994661.0589363792862991No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG891670.9492910153401306No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA848650.9034910002225058No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT750920.7994455451447406No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC494860.5268385746422073No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT451060.48020815478744305No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC375320.39957372556826837No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT326060.3471304725535265No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA263240.28025095257004945No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC256180.2727347250774778No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG238760.25418901928135923No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT211270.2249225754044763No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT199150.21201936333507576No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA185470.19745534179139596No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG178770.190322378023658No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT167620.17845184876839265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161770.17222381323984537No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC161150.17156374793596516No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC154430.1644094917390698No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG132810.1413923758198916No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG128430.13672933383441516No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC124910.13298186630270806No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC116320.12383676797959332No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG112570.11984443751257584No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT108130.11511751823962713No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT96340.10256563125132412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2461950.084.733341
CGTTATC2488700.083.709872
GCGGTAT33100.081.402391
CACGCTG105500.080.687161
CGGTATC33100.080.632032
TTATCCA2605650.079.983264
ATCCACC2688850.078.815486
GTTATCC2698200.077.306363
TATCCAC2756650.076.648845
TCCACCT2759050.076.636087
GCGTTAG3500.076.49771
CACCTTC2771000.076.2611169
CCACCTT2864500.073.7689068
GCGTTAA7050.073.543891
GCGTGAT10250.073.388041
CGTGATC10200.072.914482
CGTTAAC6500.071.920912
CGTTAGC3500.071.640712
CGTAATC5800.071.0754552
CCACCTA12300.069.103248
Produced by FastQC (version 0.11.5)