FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133934
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC58450.018.8865171
GGCGACT54250.018.664811
GATGAAA57900.018.5716921
ATGCGAT52550.018.3051871
TTTACTG58900.018.2003251
GCGCGAT37800.018.1981
TGATTTC46450.018.1974681
GATGCTA49300.018.1277791
CAATGGA58700.018.0636358
ACTGATG41950.018.0506111
GAATGAA49100.018.0447061
TCTATTG63300.017.995841
GGGACCT42250.017.8703421
TGCACCT69800.017.8495161
ACTTGAC33650.017.84729610
GGTATTG64050.017.7851141
AGCTTGA43200.017.5792681
TATTGAG35600.017.5546593
GGATTAC39700.017.4379561
CCTACCA48900.017.2857341
Produced by FastQC (version 0.11.5)