FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9569562
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT1203400.021.8701971
AGAGTGG847850.021.8465181
GCAATTG977850.021.8307042
GTTCTCG821300.021.8107221
CTAACTT749450.021.7998261
AGTGAGG681350.021.7506661
TCTACTT1210150.021.7260512
CTCGGTA808000.021.7223854
GCAGTTA519600.021.6918181
AGTTATC513450.021.6787533
CGGTACC809600.021.6753796
TCGGTAC809850.021.6727625
GTACCCA810050.021.6726198
GGTACCC809800.021.66987
GGCAATT993650.021.6555061
TCTCGGT810000.021.6510943
CTACTTC1217500.021.643233
TAACTTC750950.021.6197092
TTCTCGG805350.021.6153852
ACTTCAT1221200.021.6127835
Produced by FastQC (version 0.11.5)