FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16713088
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC59800.019.3454281
GATGAAA58600.018.7460481
TTTACTG58200.018.4209821
GGGACCT43800.018.4207231
GGCGACT57800.018.3961141
TGATTTC48000.018.3911951
TCTATTG65750.018.2476751
GAATGAA50200.018.1991541
AGCTTGA42000.018.1618771
GACTAAC42750.018.049231
ATGCGAT53400.017.9124641
TATTGAG38900.017.8757233
GTAACTG52500.017.8211781
TGCACCT70350.017.8211781
ACTGATG45400.017.795781
GGTATTG71650.017.7589971
CCTACCA56300.017.7522851
GATGCTA51800.017.7220151
GCGCGAT37800.017.70471
CCGTACT37150.017.510781
Produced by FastQC (version 0.11.5)