FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005396280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005396280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9075726
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACT1151350.021.8694041
AGAGTGG805850.021.8583341
GTTCTCG766700.021.8209551
GCAATTG946850.021.8097692
CTAACTT721400.021.7761461
GCAGTTA476950.021.7673571
TCTACTT1156900.021.742622
AGTGAGG625050.021.7310351
CTCGGTA755350.021.6913224
GTACCCA754850.021.6918
AGTTATC473000.021.6840153
GGCAATT960600.021.6785141
CGGTACC754650.021.6735466
TCGGTAC755200.021.6650455
CTACTTC1164050.021.6534863
GGTACCC755500.021.6519537
CAGTTAT473500.021.6402072
ACCCAAT756000.021.6390910
ACTTCAT1167900.021.628025
ATCATTC1170650.021.62595610
Produced by FastQC (version 0.11.5)